Lung cancer is the leading cause of malignancy-related mortality and lung adenocarcinoma accounts for about 40% of lung malignancies. The aim of this study was to investigate the associations of intraflagellar transport protein 20 (IFT20) and Golgi matrix protein 130 (GM130) expression with clinicopathological features and survival in patients …

Cell pathology in lysosomal storage diseases is characterized by the formation of distended vacuoles with characteristics of lysosomes. Our previous studies in mucopolysaccharidosis type IIIB (MPSIIIB), a disease in which a genetic defect induces the accumulation of undigested heparan sulfate (HS) fragments, led to the hypothesis that …

Introduction. The Golgi apparatus has a unique flattened and stacked cisternal structure that is conserved in most eukaryotic cells from animals to plants [].In vertebrate cells, the Golgi stacks are assembled near the centrosome and laterally connected to form a ribbon-like structure [2-4].The structure is thought to facilitate correct …

Flow cytometry analysis of GM130 was done on HeLa cells. Cells were fixed, permeabilized and stained with a GM130 rabbit polyclonal antibody (Product # PA1-077, green histogram) or a rabbit IgG isotype control (Product # MA5-16384, black histogram) at a …

Gene Summary. Golgi matrix protein 130 kD ( GM130) encodes a protein involved in Golgi compartmental organization and regulation of dendrite morphogenesis. [Date last reviewed: ] ( FlyBase Gene Snapshot )

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Inappropriate hERG localization may be an underlying cause of long QT syndrome, a hereditary and potentially fatal cardiac arrhythmia (4). Further, GM130 was implicated in signal transduction regulating invasion, migration, and cell polarization via its interaction with and activation of serine/threonine kinases YSK1 and Mst4 (5).

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Intracellular phosphatidylserine is essential for retrograde membrane traffic through endosomes Yasunori Uchidaa,1, Junya Hasegawaa,1, Daniel Chinnapenb, Takao Inouea, Seiji Okazakic, Ryuichi Katoc, Soichi Wakatsukic, Ryo Misakid, Masato Koikee, Yasuo Uchiyamae, Shun-ichiro Iemuraf, Tohru Natsumef, Ryusuke Kuwaharag, Takatoshi …

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AKAP450 localisation and dynamics in normal cells. The polyclonal antibody A24 raised against peptides encoded by exons 24–27 of AKAP450 cDNA (Keryer et al, 2003b) revealed the centrosome and a network congruent with the GA in RPE1 cells (Figure 1A).By western blotting (WB), A24 antibody recognised a major high molecular weight …

GM130 Antibody (MA5-35107) in WB. Western blot was performed using Anti-GM130 Recombinant Rabbit Monoclonal Antibody (ARC0589) (Product # MA5-35107) and a 130 kDa band corresponding to GM130 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), Hep G2 (Lane 2), HEK-293 (Lane 3), U-2 OS …

GM130 will therefore contribute to maintain the balance between Cdc42 and Ras signaling. When GM130 is lost, the cell cannot migrate persistently and there is an imbalance between Cdc42 and Ras signaling. ( B) Box Plots of two studies 32,33 comparing the mRNA levels of GM130 in normal tissues and in breast cancer tissues (obtained from …

This is a Validated Antibody Database (VAD) review about human GM130, based on 193 published articles (read how Labome selects the articles), using GM130 antibody in all methods.It is aimed to help Labome visitors find the most suited GM130 antibody. Please note the number of articles fluctuates since newly identified citations …

Maturation and post-translational modification of proteins occurs after their biosynthesis at the endoplasmic reticulum and their transport through the Golgi apparatus.

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Introduction. The Ras superfamily of small GTPases is composed of five families and Rho GTPases are one of these families that comprises at least 22 members. 1 Except few members, the major part of Rho family GTPases acts as molecular switches that cycle between the active (GTP-bound) and inactive (GDP-bound) state. 1,2 Activation of …

INCREMENTAL LINEAR SCALES TGM130 Optoelectronic 40 20 10 5 124 816 m 1 2 m 5 1 0 TGM 114, TGM 130, TGM 170 t S V /mni m 0, 5 µ m Sine wave woltage signals 1 V pp SV (remark: for details see Electrical DATA on page 28 20

Globotriaosylceramide (Gb3) is a well known receptor for Shiga toxin (Stx), produced by enterohemorrhagic Escherichia coli and Shigella dysenteriae. The expression of Gb3 also affects several diseases, including cancer metastasis and Fabry disease, which prompted us to look for factors involved in its metabolism. In the present study, we isolated two …

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Emerging evidence suggests that GM130 is involved in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. This creates the potential for novel Golgi-targeted drugs and treatments for various diseases including glycosylation defects, immune diseases, and cancer.

Abbreviations. FRAP, fluorescence recovery after photobleaching. IDR, intrinsically disordered regions. The Golgi has long been an object of fascination for cell biologists and still holds many mysteries. Discovered in the late 19th century by Camillo Golgi as a portion of the cell that occasionally took up heavy metal stains, it provided a …

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The Golgi is surrounded by a ribosome-excluding matrix. Recently, we reported that the cis-Golgi-localized golgin GM130 can phase-separate to form dynamic, liquid-like condensates in vitro and in vivo.Here, we show that the overexpression of each of the remaining cis (golgin160, GMAP210)- and trans (golgin97, golgin245, GCC88, …

Gastric cancer is one of the most common causes of digestive tract tumor. Despite of recent advances in surgical techniques and development of adjuvant therapy, the underlying mechanisms of gastric cancer remain poorly understood and relevant insight into novel treatment strategies using gene target remains incomplete.

Emerging evidence suggests that GM130 is involved in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed …

J Pharmacol Sci 108, 1 – 6 (2008)1 Journal of Pharmacological Sciences ©2008 The Japanese Pharmacological Society Current Perspective Endothelial Progenitor Cells: Therapeutic Target for Cardiovascular Diseases Takashi Umemura1 and Yukihito Higashi1,* 1 Department of Cardiovascular Physiology and Medicine, Hiroshima …

Tubulovesicular trafficking in the exocytic and endocytic pathways is regulated by members of the Rab GTPase family, which now comprises over 50 proteins 1-3.Rab proteins are members of the Ras superfamily of small molecular weight GTPases that function as molecular switches by cycling between inactive GDP-bound and active GTP-bound …

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